Detection of alkaline phosphatase (AP) using MUP as a substrate and a Turner Designs Fluorometer to measure the highly-fluorescent enzymatic product, 4MU, enables researchers to quantitate as little as 1 x10-7 mg/ml (200 pg) 4MU and has a linear range of over 5 orders of magnitude. The sensitivity of the method is about 2 µg/ml alkaline phosphatase.

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