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How do you best calibrate with a liquid?
Two things that can dramatically influence the calibration of the Cyclops are a reflective surface that is under the glass beaker and micro-bubbles on the optic surface of the Cyclops. A measurement in DI water that is < 200 mV is still ok to use as a blank.  A 200mV measurement would still represent less than 4% of the high scale.  The Cyclops should always be more than 3" away from the bottom of the beaker.  You should not try to calibrate in a beaker with a diameter less than 5", unless you are calibrating with the shade cap on the Cyclops.  The shade cap is designed as an optical “backstop” for the sensor, and can be used in a smaller volume of water.