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What causes the AlgaeWatch to give negative readings?
There are two possible reasons that the AlgaeWatch is displaying a negative number.

1.    If the liquid that is being measured generates a response lower than original blank, you will read a negative number.  If this is the case, you may need to re-calibrate using a different blank solution.

2.    Another possible cause for negative readings might be a fouled flowcell.  The AlgaeWatch has an optical compensation that will correct for biofouling up to a certain point.  Without cleaning, biofilm can continue to grow and it will at some point become thick enough to interfere with fluorescent readings.  Clean the flowcell and recalibrate as directed in page 10 of the operation manual.  If there is heavy biofouling follow the additional cleaning instructions on page 16 of the operation manual.