Products & Accessories
Applications & Customer Care
Search - Contactenhanced_search
Case Studies
Turner Blog
Products & Updates
General Information
Contacts
Content Categories
Content Sections
What variables will effect the linearity of a sample?
Fluorescence intensity is typically directly proportional (linear) to concentration.  When a concentration is too high, light cannot pass through the sample to cause excitation; thus very high concentrations can have very low fluorescence (concentration quenching).  The fluorometer reading rises at a decreasing rate and eventually begins to decrease, even though the concentration is still increasing.  Diluting a sample 1:1 or some other convenient ratio may check linearity.  If it is linear, the reading will decrease in direct proportion to the dilution.