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What variables will effect the linearity of a sample?
Fluorescence intensity is typically directly proportional (linear) to concentration.  However, when a concentration is too high, light cannot pass through the sample to cause excitation; thus very high concentrations can have very low fluorescence.  This happens when the sample is “quenched”.  The fluorometer readings will decrease, even though the concentration is still increasing.  Diluting a sample 1:1 or some other convenient ratio will check linearity.  If it is linear, the reading will decrease in direct proportion to the dilution.