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What volume do your instruments measure?
One of the most common questions we receive is: What volume do your instruments measure?  With some of our instruments, such as the Trilogy, 10AU and AquaFluor, the volume is determined/limited by the cuvette or test tube size.  In the case of very high concentrations you will get an “OVER” error message on these instruments, meaning you have saturated the sensor and are outside the detectable range.   In this case you need to dilute your samples and do the appropriate calculations to correct the concentration of your sample.  With our Cyclops sensors, including the C3 and C6P, the answer is a little more difficult to define.  The graphic below is a simplified version of how concentration dictates the volume of water sampled.  In this example, both Cyclops are in identical beakers with equal volumes of water.  The shaded portion represents the sampled volume, which is determined by the concentration of the fluorophore present.  In example A, as a result of the low concentration, the excitation and emission light travel further through the water, resulting in a larger volume being sampled.  In example B, as a result of the high concentrations, the excitation and emission light are attenuated, resulting in a smaller volume being sampled.