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Technically Speaking,
It All Adds Up...
is a series of articles for people who want to
obtain the best possible results from their fluorometer. Last
month Technically Speaking described how to optimize your fluorometer
installation when monitoring chlorophyll a in a continuous
flow setup, using a fluorometer such as the Turner Designs AlgaeWatch.
This month's article will describe how algae buildup on the sample
cell in the AlgaeWatch impacts the displayed chlorophyll reading;
and some cleaning tips to minimize the impact of the buildup.
Determining When to Clean a Fluorometer
Sample Cell
Even if you are using the Basket Strainer on the inlet to the AlgaeWatch
Sample Cell, described in last month's article, you will still need
to clean the sample cell periodically to optimize your measurement
accuracy and repeatability. When to clean? If your fluorometer is
remotely located, then minimizing the frequency of cleaning becomes
an important issue because of the impact on operating expenses,
(time).
The following procedure will give you
an appreciation for how to optimize the schedule for cleaning the
sample cell.
Step 1 - Decide on acceptable change
in reading due to fouling buildup.
This is an arbitrary value, and will generally be a value greater
than the AlgaeWatch accuracy specification, (±3%), up to
a maximum acceptable change in reading due to buildup on the sample
cell. For this explanation, it will be assumed that the change in
reading due to sample cell fouling should be less than -10%.
Step 2 - Measure without cleaning!
To determine if the fluorometer sample cell needs cleaning, start
by injecting some calibration solution and noting the reading -
be sure that you don't clean the cell before making this first reading,
since the value will be used as a benchmark.
Step 3 - Clean the Sample Cell
Clean the sample cell by inserting the accessory cleaning brush
for the AlgaeWatch, and vigorously clean the cell. (The cell is
located behind the desiccant plug on the sample chamber, and can
be used to confirm how far to insert the brush when cleaning). Continue
brushing until the cleaning brush has removed all the debris and/or
fouling, (equal to the bristles being clean when the brush is removed
).
Step 4 - Measure the calibration
solution
Using the same calibration solution as in Step 2, inject a sample
of the remaining solution until the reading stabilizes again to
within ±0.2. Note the new reading of the same solution. If
this new reading differs from the reading obtained in Step 2 by
more than the arbitrarily established value of 10%, then the time
from the last cleaning was too long, and appropriate adjustments
to the cleaning schedule should be made, (reduce the cleaning interval).
Less than 10% change means that all
the data collected since the last cleaning of the sample cell is
within the arbitrarily established limits of Step 1.
Note that it is unlikely that fouling
buildup has a known relationship with time, (and it will vary with
the seasons, etc.). This means that it is not possible to determine
a correction factor for the fluorometer readings for the time back
to the last cleaning.
To reduce the chance of having to discard
data, it is better to clean the sample cell as frequently as possible,
noting the change in reading after cleaning, and making appropriate
changes to the cleaning schedule.
Next month's article will discuss how
the environment affects the measurement of chlorophyll. For more
information on cleaning the sample cell, view
the video.
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