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Nitrate Analysis


Nitrate is taken up by phytoplankton, algae, or plants as a primary source of nitrogen and reduced to help build nucleotides, building blocks of proteins and enzymes. Although nitrate is the most abundant form of fixed nitrogen in aquatic systems with an average concentration of 30 micromolar, it is typically limiting in surface waters due to algal uptake. Nitrate is typically accumulated at depth, below the photic zone, unless it is introduced by rainfall or upwelling events. Other sources that introduce nitrate to surface waters are leaking sewage systems, release from tissues of dead or decaying plants or animals, and excretory material. Algal blooms usually follow the increase of nitrate concentrations in surface waters. Researchers analyze nitrate concentrations in an effort to better explain phytoplankton blooms.


  • Introduction to Nitrate/Nitrite and Methods (S-0091)
  • Comparison Data (S-0092)

  • Strickland and Parsons 1968 Procedure (S-0093)

  • LaMotte Test Kit Procedure (S-0094)
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