Includes two 20 mL ampoules with known concentration of Chlorophyll a in a 90% acetone solution
10-850 Chlorophyll a Standards
Chlorophyll powder isolated from Anacystis nidulans is dissolved in 90% acetone and spectrophotometrically analyzed using Jeffrey Method (1997) to determine concentration of parent stock solution. Parent stock solution is diluted to create two chlorophyll standard solutions, B and C. Fluorescence signal of the resulting solutions is checked to confirm accuracy of dilution and twenty milliliters of each chlorophyll solution, B and C, is sealed in its own ampoule and stored in the dark at temperatures below -20° Celsius.
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