SCUFA Submersible Fluorometer
Effective December 1, 2008 we obsoleted our SCUFA™ (Self-Contained Underwater Fluorescence Apparatus) Submersible Fluorometer. Although there was a solid customer base, over the years we had received numerous requests for product enhancements which were too costly to make on the SCUFA™. Accordingly, in July 2008 we introduced the new C3™ Submersible Fluorometer. Some of the most major enhancements we included on the C3™ are:
• Configurable with 1, 2 or 3 optical kits
• Optional, integrated wiper & pressure sensor
• All plastic housing to minimize corrosion
Frequently Asked Questions
Can I use Rhodamine WT as a secondary Standard for the SCUFA?
I inherited a SCUFA, are instructions for the Flow Cap and specifications available?
View SCUFA Flow Cap Instructions
I lost the protective cover (aka dummy plug) for the data and power pins on my SCUFA, is this part still available?
How do I attach the SCUFA to a CTD?
Two pieces of hardware are required to integrate a SCUFA with a CTD - an integration cable and a mounting bracket. If possible, the integration should be conducted by the CTD manufacturer. If this is not possible, you must contact the appropriate cable/connector vendor to have an integration cable made that will allow communication between the fluorometer and CTD. An integration cable consists of two in-line connectors, locking sleeves and a cable of specified length, usually 3-4 feet.
The in-line connector required for the SCUFA is an Impulse 8-pin, female connector (Impulse P/N: MIL-8-FS), the locking sleeve is P/N MCDLS/F. The maximum cable length is 50m.
The bulkhead connector on the SCUFA is Impulse P/N:MCBH-8-MS.
The wiring for the SCUFA bulkhead connector is as follows:
1 V Batt +
2 V Batt –
3 RS-232 ground
4 RS-232 T1 Out
5 RS-232 R1 In
6 V Out 1
7 V Out 2
What environmental factors cause error in in vivo chlorophyll analysis with the SCUFA?
Light history will have significant affects on the fluorescence in algal cells. Cells will fluoresce more chlorophyll per cell when in darker environments than in well lit zones. One way of reducing the effects of light is to use the flow-through cap when sampling natural waters. By using a flow-through cap and an external pump, the algal cells will be dark-adapted before entering the fluorometer, significantly reducing fluorescence error caused by variations in the light history of the cells.
Dissolved organic matter (DOM), chlorophyll degradation products, and turbidity can also affect fluorescence response. If these factors are suspected to be significant it is worth conducting a quick study to look at the effects by comparing the fluorescence from filtered and non-filtered water samples from below the photic zone where chlorophyll concentrations would be at a minimum.
Are there some steps I can take to troubleshoot my SCUFA?
The following are some common problems and solutions to diagnose your SCUFA:
Possible Solution: Check your PC setting to make sure that the correct COM port is selected. Check wiring between the PC and SCUFA to make sure that everything is connected correctly. Check to see that the SCUFA is powered.
Possible Solution: Check your connections to the SCUFA and refer to Appendix D of the manual.
Possible Solution: To check calibration refer to page 11 of the SCUFA manual regarding recalibration.
Possible Solution: Turn off internal logging.
Possible Solution: Reset your PC time and date. This is transferred from your PC while linked to the SCUFA.
Possible Solution: Rinse the optics with fresh water and dry with Kim wipes or non-abrasive lens paper.
My SCUFA will not enter "sleep" mode after the IDL has been configured.
What volume of liquid will the SCUFA Flowthrough Cap hold?
The SCUFA Flowthrough Cap will hold about 40mL of fluid. If you are interested in finding a minimum sample volume, you must also include enough to account for tubing area.
Is the SCUFA's serial data temperature compensated?
If you purchased the temperature compensation package with your SCUFA, then as long as it is enabled (thermometer icon is active in SCUFA soft), the fluorescence output will be temperature compensated. If the thermometer icon is not active then your readings will be uncompensated.
Can you provide an example of SCUFA Serial data output?
e following data set is an example of the RS232 serial data output captured by a serial data acquisition program. In this case, the Hyperterminal program that is included with the Windows operating system was used to save the data.
Note: The TCF item below stands for “Temp. Corrected Fluorescence” using a configurable Temperature Coefficient entered into the SCUFA. In this case the coefficient value was set to zero, resulting in no correction.
08/08/01 16:15:50: 18.898 18.898 83.289 26.3(C)
08/08/01 16:15:51: 18.896 18.896 83.673 26.2(C)
08/08/01 16:15:52: 18.980 18.980 82.341 26.2(C)
08/08/01 16:15:53: 18.950 18.950 80.537 25.7(C)
08/08/01 16:15:54: 18.947 18.947 80.523 25.0(C)
08/08/01 16:15:55: 18.952 18.952 80.525 24.4(C)
08/08/01 16:15:56: 18.949 18.949 80.525 23.8(C)
08/08/01 16:15:57: 18.939 18.939 80.516 23.3(C)
08/08/01 16:15:58: 18.945 18.945 80.521 23.1(C)
08/08/01 16:15:59: 18.951 18.951 80.525 22.8(C)
08/08/01 16:16:00: 18.944 18.944 80.527 22.7(C)
What is the range on the SCUFA solid secondary standard in Rhodamine WT equivalence?
What is the sensitivity of the SCUFA?
The SCUFA sensitivity is defined in terms of the minimum detection limits of various analytes.
The SCUFA can detect:
Chlorophyll 0.02ppb or 0.02 μg/L
Rhodamine WT 0.04ppb or 0.04 μg/L
Do I adjust the sensitivity with a gain knob or switch?
Is SCUFAsoft compatible with Macintosh computers?
No, SCUFAsoft is not compatible with Macintosh computers.
How often do I need to calibrate the SCUFA?
What is the purpose of the solid secondary standard?
The solid secondary standard is designed for recalibration in place of primary standards and to check for instrument performance and drift. It is very stable and does not require special storage conditions.
What is the concentration of the solid secondary standard?
There is no exact concentration for the solid secondary standard. It is a relative concentration for both Chlorophyll and Rhodamine WT. You can easily adjust the fluorescence signal of the solid standard and use it as a reference value for future calibrations as well as check for electronic drift.
What is the power consumption if using internal data logging?
How long can the SCUFA stay submerged with anti-fouling screens?
How do I set the 0-5V outputs?
Activating the analog signal output should follow instrument calibration. By calibrating first, you can then set the 0V and 5V to calibrated values. For example, if you calibrated with a 10ppb solution and know that you will not exceed 100ppb in the field, you can set 5V to equal 100. By doing this you can optimize the resolution and accuracy of your analog data and interpret your analog data with a calibration coefficient. In this example, the calibration coefficient would be 20 (5V = 100ppb, 0.5V = 10ppb).
Once set, the analog output will be activated upon the next power up as long as the unit is not connected to the portable computer.
What is the Flowthrough cap for?
How can I use the SCUFA in the laboratory?
How does the SCUFA detect and quantitate chlorophyll in water?
Could I calibrate with extracted chlorophyll?
Is there any maintenance that you recommend for the SCUFA?
What are the options for Internal Datalogging (IDL) configurations for the SCUFA and what is the total time available?
Click here for a reference chart.